| | Barley (Hordeum vulgare) grain contains significant levels of phosphorous, though most is bound in the form of phytic acid (phytate) and is nutritionally unavailable to monogastrics. Recently USDA researchers, utilizing mutation breeding, have developed low phytate barley mutants which have increased available phosphorous. Of several low phytate mutants, the lpa1-1 locus has been localized to the long arm of chromosome 2H. An RFLP marker located in this region (ABC153) was successfully converted to a dominant SCAR marker. The doubled haploid population from a cross between CDC McGwire and the low phytate mutant Hvlpa1-1 was screened to confirm linkage with the converted marker. A second low phytate mutant, M2 635, was also examined with the ABC153 SCAR marker. It was determined that M2 635 is not allelic to Hvlpa1-1. An ISSR marker linked to M2 635, termed LP75, has been mapped to chromosome 1H. This low phytate mutant has been renamed Hvlpa3-1. The SCAR marker ABC153 was also polymorphic in an additional cross involving a third mutant, M2 955. Initial results suggest that M2 955 and Hvlpa1-1 are not allelic and screening of a larger population is in progress.
Vicky Roslinsky1, Victor Raboy2, Brian G. Rossnagel1, and Graham J. Scoles1
1 Dept. of Plant Sciences/Crop Development Centre, University of Saskatchewan, Saskatoon, SK, S7N 5A8
2 USDA-ARS, Aberdeen, ID, 83210 USA
Presented at the 3rd Canadian Barley Symposium, June 19-20, 2003 |
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