| | Our objective was to develop a calibration equation to predict the deoxynivalenol (DON) levels in barley using near-infrared spectroscopy. A FOSS NIR Systems 6500 Near Infrared Spectrometer was used to collect the spectra of 577 samples grown at Brandon and Ottawa in 2001 with DON levels ranging from 0 to 161 ppm. The DON levels of these samples were measured using the ELISA test. The computer programs CENTER and SELECT were used to define the population boundaries of these samples and to select those samples to be used for calibrations. Population boundaries were established with a maximum standardized H distance from the average spectrum of 3. Calibration samples were selected with a minimum standardized H distance between samples of 0.6. Calibrations were developed using different math treatments with multiplicative scatter correction (de-trend). This equation was used to predict the DON levels in sample sets grown at Brandon in 2002. The results show that this equation has the potential to be used as a screening tool to eliminate those samples with high levels of DON.
Arganosa, G.C.1, B.G. Rossnagel1, B.G. Legge2, T. Zatorski1 and J. Tucker2
1 Crop Development Centre, University of Saskatchewan, Saskatoon, SK, S7N 5A8
2 Agriculture and Agri-Food Canada, Brandon Research Station, Brandon, MB, R7A 5Y3
Presented at the 3rd Canadian Barley Symposium, June 19-20, 2003 |
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